Removal of RNA primers and filling in the gaps. Product Source An E. Pin On Biostuffs DNA Polymerase I E coli is a DNA-dependent DNA polymerase with inherent 3 5 and 5 3 exonuclease activities 1. . DNA synthesis and proofreading. Unwinds the double helix by breaking hydrogen bonds. There are several forms of DNA polymerase that play a role in DNA replication and they usually work in pairs to copy one molecule of double-stranded DNA into two new double stranded DNA molecules. DNA ligase then forms ______ bonds between DNA Okazaki fragments. The function DNA polymerase includes. B It can synthesize DNA in the 3 to 5 direction. Builds an RNA primer on leading and lagging strands. 05 point removing RNA and replacing it with dNTPs primary DNA replication enzyme in the polymerization of nucleotides initiation of DNA synthesis removing a nucleotide that forms a mismatched base pair covalently joining two strands ...